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Synthesis of tumour necrosis factor-alpha and interferons by mononuclear cells from Theileria annulata-infected cattle

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Articles & Journals
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Agricultural Value Chains / Agri-Businesses
Health & Diseases
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Biological & environmental
Boid, R.; Brown, C.G.D.; Entrican, G.; Preston, P.M.; Richardson, W.

Bovine macrophage-derived tumour necrosis factor-alpha/ cachectin (TNF-a) was synthesized when peripheral blood mononuclear cells (PBMC) and purified adherent PBMC from na-ve and Theileria annulata-infected cattle were incubated in vitro with concanavalin A (Con-A) or bovine recombinant interferon gamma (Bo rIFN–). TNF-a production was also induced when adherent PBMC were cultured with T. annulata macroschizont-infected cells. In contrast, non-adherent PBMC from sublethally infected cattle produced interferon (IFN) when incubated with Hu rIL-2, Con-A, phytohaemagglutinin (PHA) or T. annulata macroschizont-infected cells growing as cell lines in vitro. Whilst PBMC from lethally infected cattle spontaneously produced IFN– during advanced stages of infection, the sera of such animals contained type 1 IFN (alpha/beta). IFN was also produced by T. annulata macroschizont-infected cell lines maintained in vitro. This work suggests that cytokines serve as crucial links between proliferating Theileira-infected cells and the characteristic clinical symptoms of tropical theileriosis.