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PCR-based detection and characterization of Pseudomonas solanacearum for use in less developed countries

Published by:
Publication date
27/03/1995
Language:
English
Type of Publication:
Articles & Journals
Focus Region:
Global
Focus Topic:
Land / Water / Resource Management
Type of Risk:
Biological & environmental
Commodity:
Crops
Source
http://www.dfid.gov.uk/R4D/Output/187483/Default.aspx http://dx.doi.org/10.1111/j.1365-2338.1995.tb01462.x
Author
Seal, S.

Pseudomonas solanacearum-specific DNA sequences were identified by 16S rRNA sequencing and genomic subtraction experiments. Oligonucleotide primers for these regions were constructed allowing single cell detection by PCR amplification. These specific PCR tests are being adapted for use in less sophisticated laboratories in Asia and Africa.