Nucleotide sequence of the rDNA internally transcribed spacer (ITS) 1 of twelve isolates of Colletotrichum, originally identified as either C. fructigenum, C. gloeosporioides or C. musae, was determined. Comparison of the sequence data with that from previously characterized isolates of Colletotrichum species revealed 97-100% homology with C. acutatum and only 79-81% homology with C. gloeosporioides and C. musae. We conclude that the isolates belong to C. acutatum. The value of sequence data from ITS 1 in species assignment is demonstrated.